fitc anti rat cd45 antibody  (Elabscience Biotechnology)

Journal: Genes & Diseases

Article Title: The bidirectional effects of APPswe on the osteogenic differentiation of MSCs in bone homeostasis by regulating Notch signaling

doi: 10.1016/j.gendis.2024.101317

Figure Lengend Snippet: APPswe inhibits the self-renewal, proliferation, and migration of MSCs. (A, B) The effect of APPswe on the self-renewal ability of MSCs was detected by colony-forming unit-fibroblast assays. (C, D) Cell cycle changes in MSCs treated with APPswe were detected by flow cytometry. (E) The expression of the proliferation-related gene Ki67 in different groups was detected by cell immunofluorescence. (F) The expression levels of proliferation-related proteins in MSCs after treatment with APPswe were detected by western blotting. (G) Transwell assays were used to detect the effect of APPswe treatment on the cell migration ability of MSCs. (H) The effect of APPswe on MSC migration was detected by a wound healing test. (I) The expression levels of F-actin in the different treatment groups were detected by FITC-phalloidin staining. (J) Western blot analysis was performed to detect the expression levels of migration-related proteins in the different groups. n = 3; ∗ p < 0.05; ∗∗ p < 0.01, ∗∗∗ p < 0.001. APPswe, Swedish mutant amyloid precursor protein; MSC, mesenchymal stem cell.

Article Snippet: FITC anti-rat CD45, PE anti-rat CD90, and PE anti-rat CD29 antibodies were obtained from Elabscience Biotechnology (Wuhan, China).

Techniques: Migration, Flow Cytometry, Expressing, Immunofluorescence, Western Blot, Staining, Mutagenesis

Journal: Genes & Diseases

Article Title: The bidirectional effects of APPswe on the osteogenic differentiation of MSCs in bone homeostasis by regulating Notch signaling

doi: 10.1016/j.gendis.2024.101317

Figure Lengend Snippet: Effects of APPswe-C on the proliferation and migration of MSCs. (A, B) The effect of APPswe-C on the self-renewal of MSCs was detected by a colony-forming unit-fibroblast assay. (C, D) The effect of APPswe-C on the cell cycle was detected by flow cytometry. (E) The expression of the proliferation gene Ki67 was detected by cellular immunofluorescence. (F) The expression levels of proliferation-related proteins after APPswe-C treatment were detected by western blotting. (G, H) The cell migration of each group after APPswe-C treatment was detected by Transwell assay. (I, J) The effect of APPswe-C treatment on cell migration was detected by a wound healing test. (K) The expression of F-actin in the cells of each group after APPswe-C treatment was detected by FITC-phalloidin staining. (L) The expression levels of cell migration-related proteins were detected by western blot. MSCs were treated with APPswe-C for 24 h and then with BMP2 for 48 h to detect the expression levels of related proteins. n = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. APPswe-C, Swedish mutant amyloid precursor protein without an intracellular segment; MSC, mesenchymal stem cell; BMP2, bone morphogenetic protein 2.

Article Snippet: FITC anti-rat CD45, PE anti-rat CD90, and PE anti-rat CD29 antibodies were obtained from Elabscience Biotechnology (Wuhan, China).

Techniques: Migration, Flow Cytometry, Expressing, Immunofluorescence, Western Blot, Transwell Assay, Staining, Mutagenesis

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: CD8 + T cells promote tubule-interstitial damage in malaria-induced acute kidney injury

doi: 10.3389/fcimb.2025.1561806

Figure Lengend Snippet: Adoptive transfer of malaria-responsive T cells induces an increase in CD8 + T cells population in renal tissue. (A) Dot-plot representing the percentage of CD4 and CD8 + T cells in total CD45 + renal cells of the naive → naive (orange) and infected → naive (blue) groups. (B) Quantification of the percentage of CD4 + (n = 5) and (C) CD8 + T cells (n = 5) in the kidney. (D) Representative immunoblotting of cortical perforin-1 expression in renal tissue. (E) Quantification of perforin-1 expression in renal tissue (n = 4). *versus naive → naive group, P < 0.05.

Article Snippet: The cells were incubated with the following antibodies for surface markers: PeCy5.5-conjugated rat IgG2a anti-murine CD8 (45-0081-80, Thermo Fisher Scientific, Waltham, MA), PE-conjugated rat IgG2b anti-mouse CD4 (clone GK1.5; 11-0041-82, Thermo Fisher Scientific), and FITC-conjugated rat IgG1 anti-mouse CD45 (11.0452-82, Thermo Fisher Scientific).

Techniques: Adoptive Transfer Assay, Infection, Western Blot, Expressing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: CD8 + T cells promote tubule-interstitial damage in malaria-induced acute kidney injury

doi: 10.3389/fcimb.2025.1561806

Figure Lengend Snippet: Depletion of CD8 + T cells induces an increase in parasitemia but does not change the percentage of CD4 T cells. (A) Representative scheme of CD8 + T cell depletion. Animals were infected with 10 6 iRBCs and treated with anti-mouse CD8 antibody on the day 2 and 3 post infection. The analyses were performed on day 5. (B) Peripheral parasitemia was assessed daily (n = 4). (C) Histogram representing the percentage of CD4 + and CD8 + T cells in CD45 + cells of the spleen. (D) Quantification of the percentage of CD4 + and CD8 + T cells in spleen (n = 4). (E) Histogram representing the percentage of CD4 + and CD8 + T cells in CD45 + cells of the kidney. (F) Quantification of the percentage of CD4 + and CD8 + T cells in spleen (n = 4). *versus undepleted group, P < 0.05.

Article Snippet: The cells were incubated with the following antibodies for surface markers: PeCy5.5-conjugated rat IgG2a anti-murine CD8 (45-0081-80, Thermo Fisher Scientific, Waltham, MA), PE-conjugated rat IgG2b anti-mouse CD4 (clone GK1.5; 11-0041-82, Thermo Fisher Scientific), and FITC-conjugated rat IgG1 anti-mouse CD45 (11.0452-82, Thermo Fisher Scientific).

Techniques: Infection

Journal: STAR Protocols

Article Title: Protocol for isolation of endothelial cells from adult mouse brain

doi: 10.1016/j.xpro.2025.103837

Figure Lengend Snippet: FACS gating strategy for EC isolation from a whole brain sample Representative FACS plots for the gating strategy to sort ECs from a whole brain sample, related to Step 78. One whole brain from a six-month-old male C57BL/6J mouse (Jackson Laboratory, strain #: 000664) was used to generate this sample. (A) Forward scatter area (FSC-A) vs side scatter area (SSC-A) to exclude debris. (B) Side scatter height (SSC-H) vs side scatter width (SSC-W) to exclude doublets. (C) Forward scatter height (FSC-H) vs forward scatter width (FSC-W) to exclude doublets. (D) Negatively select for DAPI + dead cells, CD13 + mural cells (BV421), and CD11b + /CD45 + microglia/macrophages (FITC). (E) Sort for CD31 + endothelial cells (AF647). (F) Unstained control.

Article Snippet: Rat anti-ms CD45 FITC clone 30-F11 (dilution: 1:167) , eBioscience , Cat#11-0451-85; RRID: AB_465051.

Techniques: Isolation, Control